dacA [2019-03-19 19:42:28]

penicillin-binding protein 5, major D-alanyl-D-alanine carboxypeptidase

locus: BSU00100

pI: 5.67

mw: 48.47 kDa

protein length: 443 aa

gene length: 1332 bp

function: carboxypeptidase

product: penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 17,534 18,865

The protein

Catalyzed reaction/ biological activity

• Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala (according to Swiss-Prot)

Protein family

• peptidase S11 family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|F7AD78F9AB98A150E8CDFC1D01A9F938FF9F8BD1|DacF]

Structure

• [PDB|1XP4] (from Streptococcus pneumoniae, 37% identity) [pubmed|15596446]

[SW|Localization]

• secreted (according to Swiss-Prot)
• membrane associated [Pubmed|18763711]
• during vegetative growth: septal, distinct spots at periphery [pubmed|14731276]

Expression and Regulation

Operons

• genes: [gene|7C3081BC8D416127A881627EB56C0628359111CF|dacA]-[gene|651D1E534490276B9BE7BFE7B5819176AE09891E|pdxS]-[gene|6F96C10759C1C20B3A6B396E3718F0A9281F100A|pdxT]-[gene|82B92D2FB8DB88689D3A8B77FAD2F63FCCA04C7E|serS]
  description: [Pubmed|22383849]

  regulation

  • the leader mRNA is processed upstream of [gene|7C3081BC8D416127A881627EB56C0628359111CF|dacA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]

  Additional information

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]∼P binds to the promoter region, but regulation has not been explored [pubmed|25666134]

Biological materials

Mutant

• 1A742 ( ''dacA''::''cat''), [Pubmed|3087956], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A742&Search=1A742 BGSC]
• 1A743 ( ''dacA''::''cat''), [Pubmed|3087956], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A743&Search=1A743 BGSC]
• BKE00100 ([gene|7C3081BC8D416127A881627EB56C0628359111CF|dacA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCGTACGACCTCCGTAT, downstream forward: _UP4_TAATCAATTGAAAGAGCTCT
• BKK00100 ([gene|7C3081BC8D416127A881627EB56C0628359111CF|dacA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCGTACGACCTCCGTAT, downstream forward: _UP4_TAATCAATTGAAAGAGCTCT

GFP fusion

• 2085 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|7C3081BC8D416127A881627EB56C0628359111CF|dacA]::pSG1493 (cat P_xyl-gfpa-[gene|7C3081BC8D416127A881627EB56C0628359111CF|dacA]1-423) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

References

Reviews

• 18266855

Original publications

• 9864321,18763711,14731276,10383963,23531131,23199363,26499795,15596446,29794222